Tibidabo Scientific LIFA FLIM vTAU DemoTibidabo Scientific LIFA FLIM vTAU Demo

FLIM Detection of cAMP Signaling
Detection of cAMP signaling in isoproterenol-stimulated HeLa cells expressing a EPACS-H189 biosensor (AddGene Plasmid 170350)

From October 15 to November 15 Micrographia@Western will be hosting a demonstration of a Tibidabo Scientific LIFA FLIM vTAU system. This Fluorescence Lifetime Imaging Microscope (FLIM) detects the unique temporal signature of fluorophores to provide several unique imaging approaches:

  • Detection of metabolic and signaling processes such as mitochondrial polarization and cAMP signaling.
  • Separation of fluorophores from autofluorescence in challenging samples such as tissue sections.
  • “Label-free” detection of some tissue components based on their intrinsic autofluorescence signature.
  • Fluorescence Resonance Energy Transfer (FRET) detection of inter-protein interactions between fluorescently labeled proteins.
  • Separation of signals from fluorophores with similar overlapping or identical emission wavelengths.

Available Channels

  • 450 nm – CFP, mTurquoise, ATTO465, and other cyan fluorophores
  • 490 nm – eGFP, FITC, Alexa 488), and other green fluorophores
  • 636 nm – Cy5, Alexa 647, miRFP670

Seminars and Training

An educational seminar and on-line training will be available. Details on these will be posted at a later date.

Signup for Instrument Time

A signup calendar will be made available closer to the start of the demonstration.


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